Animal Tissues Quality and Quantity Requirements

Animal Tissues Quality and Quantity Requirements

We process gDNA extraction in automated processes and it is critical that you will follow our recommendations about tissue sampling, storage, packaging and shipment.

Minimise the risk that the submitted tissues contain pathogenic viruses, bacteria, pest and parasites.

The success of gDNA extraction relies on tissue quality from sampling to arrival to our facility. Please use the best methods of sampling and storing your samples prior shipment. These methods depend on the organism and tissue. If unsure, please contact us at samples@diversityarrays.com we will assist based on experience with large diversity of processed organisms/tissue sources.

Tissues other than blood

We accept 10-15mg of tissue submerged in 70% ethanol which volume cannot exceed 100µl. Please ensure the quantity of submitted tissue is as uniform as possible and be aware that additional fee will be charged if we need to subsample too large tissue samples.

If you are submitting tissues know to leave behind exonucleolitic activity (as for example fins of some fish species) please ensure you tick the box consenting for additional post extraction gDNA clean up. It might be the only way to successfully process such samples.

If you are submitting very small whole organisms as for example tiny insects, nematodes etc. it is critical that you alert us by placing adequate note while placing your order and on the Sample Tracking File in the Comments.

Blood

We accept full blood samples, ideally freshly collected and dried before shipment (3-5µl per sample). Liquid blood (5-10µl per sample) suspended under 70% ethanol or blood samples preserved on FTA paper are fine too. Please note that if you want to secure low price for DNA extraction from blood preserved on FTA paper we require the disks of 3 to 5 mm in diameter to be submitted in the recommended microtiter plate. Please note that subsampling from FTA paper performed in our facility will attract an additional fee.

Tissue samples from museum collections/valuable/prolonged storage materials

We recommend that the gDNA from museum/valuable/prolonged storage materials is extracted by our users. If you have to rely on our DNA extraction services we will do our best, however it is critical you alert us that your material is from museum collections/valuable/prolonged storage by adding adequate note while placing your order and on the Sample Tracking File in the Comments. Otherwise your samples will be processed using our standard procedures which may not be sufficient for museum collections/valuable/prolonged storage tissues.