Home
>
Technology and Resources
>
Scientific Papers and Publications

Scientific Papers and Publications

Jump to:

Mapping Agronomic and Quality Traits in Elite Durum Wheat Lines under Differing Water Regimes

Jan 2020 | Mérida-García R, Bentley A R, Gálvez S, Dorado G, Solís I, Ammar K, Hernandez P,
Agronomy. 2020. 10(1) : 144.
DOI: 10.3390/agronomy10010144 | Show abstract ...

View paper (you will be redirected to another page)

Whole-genome diversity, population structure and linkage disequilibrium analysis of globally diverse wheat genotypes using genotyping-by-sequencing DArTseq platform

Jan 2020 | Mahboubi M, Mehrabi R, Naji A M, Talebi R,
3 Biotech. 2020. 10 : 4.
DOI: 10.1007/s13205-019-2014-z | Show abstract ...

View paper (you will be redirected to another page)

Identification of molecular markers for the Pc39 gene conferring resistance to crown rust in oat

Jan 2020 | Sowa S, Paczos-Grzęda E,
Theor Appl Genet. 2020.
DOI: 10.1007/s00122-020-03533-z | Show abstract ...

View paper (you will be redirected to another page)

Identification of potential candidate genes controlling pea aphid tolerance in a Pisum fulvum high‐density integrated DArTseq SNP‐based genetic map

Jan 2020 | Barilli E, Carrillo‐Perdomo E, Cobos M J, Kilian A, Carling J, Rubiales D,
Pest Manag Sci.. 2020.
DOI: 10.1002/ps.5696 | Show abstract ...

View paper (you will be redirected to another page)

Springtail phylogeography highlights biosecurity risks of repeated invasions and intraregional transfers among remote islands

Dec 2019 | Baird H P, Moon K L, Janion-Scheepers C, Chown S L,
Evolutionary applications,. 2020. 13(5) : 960-973.
Show abstract ...

Human-mediated transport of species outside their natural range is a rapidly growing threat to biodiversity, particularly for island ecosystems that have evolved in isolation. The genetic structure underpinning island populations will largely determine their response to increased transport and thus help to inform biosecurity management. However, this information is severely lacking for some groups, such as the soil fauna. We therefore analysed the phylogeographic structure of an indigenous and an invasive springtail species (Collembola: Poduromorpha), each distributed across multiple remote sub-Antarctic islands, where human activity is currently intensifying. For both species, we generated a genome-wide SNP data set and additionally analysed all available COI barcodes. Genetic differentiation in the indigenous springtail Tullbergia bisetosa is substantial among (and, to a lesser degree, within) islands, reflecting low dispersal and historic population fragmentation, while COI patterns reveal ancestral signatures of postglacial recolonization. This pronounced geographic structure demonstrates the key role of allopatric divergence in shaping the region’s diversity and highlights the vulnerability of indigenous populations to genetic homogenization via human transport. For the invasive species Hypogastrura viatica, nuclear genetic structure is much less apparent, particularly for islands linked by regular shipping, while diverged COI haplotypes indicate multiple independent introductions to each island. Thus, human transport has likely facilitated this species’ persistence since its initial colonization, through the ongoing introduction and inter-island spread of genetic variation. These findings highlight the different evolutionary consequences of human transport for indigenous and invasive soil species. Crucially, both outcomes demonstrate the need for improved intraregional biosecurity among remote island systems, where the policy focus to date has been on external introductions.

View paper (you will be redirected to another page)

Application of DArT seq derived SNP tags for comparative genome analysis in fishes; An alternative pipeline using sequence data from a non-traditional model species, Macquaria ambigua

Dec 2019 | Shams F, Dyer F, Thompson R, Duncan R P, Thiem J D, Kilian A, Ezaz T,
PLoS ONE. 2019. 14(12) : e0226365.
DOI: 10.1371/journal.pone.0226365 | Show abstract ...

View paper (you will be redirected to another page)

Morphological and molecular characterization of freshwater prawn of genus Macrobrachium in the coastal area of Cameroon

Dec 2019 | Makombu J G, Stomeo F, Oben P M, Tilly E, Stephen O O, Oben B O, Cheruiyot E K, Tarekegn G M, Zango P, Egbe A E, Ndagyong A, Mialhe E, Ngueguim J R, Mujibi F D N,
Ecol Evol.. 2019. 00 : 1-17.
DOI: 10.1002/ece3.5854 | Show abstract ...

View paper (you will be redirected to another page)

Unlocking the Genetic Diversity and Population Structure of a Wild Gene Source of Wheat, Aegilops biuncialis Vis., and Its Relationship With the Heading Time

Nov 2019 | Ivanizs L, Monostori I, Farkas A, Megyeri M, Mikó P, Türkösi E, Gaál E, Lenykó-Thegze A, Szőke-Pázsi K, Szakács É, Darkó É, Kiss T, Kilian A, Molnár I,
Front. Plant Sci. 2019 November. 10 : 1531.
DOI: 10.3389/fpls.2019.01531 | Show abstract ...

View paper (you will be redirected to another page)

GWAS to Identify Genetic Loci for Resistance to Yellow Rust in Wheat Pre-Breeding Lines Derived From Diverse Exotic Crosses

Oct 2019 | Ledesma-Ramírez L, Solís-Moya E, Iturriaga G, Sehgal D, Reyes-Valdes M H, Montero-Tavera V, Sansaloni C P, Burgueño J, Ortiz C, Aguirre-Mancilla C L, Ramírez-Pimentel J G, Vikram P, ,
Front. Plant Sci. 2019 October. 10 : 1390.
DOI: 10.3389/fpls.2019.01390 | Show abstract ...

Yellow rust (YR) or stripe rust, caused by Puccinia striformis f. sp tritici Eriks (Pst), is a major challenge to resistance breeding in wheat. A genome wide association study (GWAS) was performed using 22,415 single nucleotide polymorphism (SNP) markers and 591 haplotypes to identify genomic regions associated with resistance to YR in a subset panel of 419 pre-breeding lines (PBLs) developed at International Center for Maize and Wheat Improvement (CIMMYT). The 419 PBLs were derived from an initial set of 984 PBLs generated by a three-way crossing scheme (exotic/elite1//elite2) among 25 best elites and 244 exotics (synthetics, landraces) from CIMMYT’s germplasm bank. For the study, 419 PBLs were characterized with 22,415 high-quality DArTseq-SNPs and phenotyped for severity of YR disease at five locations in Mexico. A population structure was evident in the panel with three distinct subpopulations, and a genome-wide linkage disequilibrium (LD) decay of 2.5 cM was obtained. Across all five locations, 14 SNPs and 7 haplotype blocks were significantly (P < 0.001) associated with the disease severity explaining 6.0 to 14.1% and 7.9 to 19.9% of variation, respectively. Based on average LD decay of 2.5 cM, identified 14 SNP–trait associations were delimited to seven quantitative trait loci in total. Seven SNPs were part of the two haplotype blocks on chromosome 2A identified in haplotypes-based GWAS. In silico analysis of the identified SNPs showed hits with interesting candidate genes, which are related to pathogenic process or known to regulate induction of genes related to pathogenesis such as those coding for glunolactone oxidase, quinate O-hydroxycinnamoyl transferase, or two-component histidine kinase. The two-component histidine kinase, for example, acts as a sensor in the perception of phytohormones ethylene and cytokinin. Ethylene plays a very important role in regulation of multiple metabolic processes of plants, including induction of defense mechanisms mediated by jasmonate. The SNPs linked to the promising genes identified in the study can be used for marker-assisted selection.

View paper (you will be redirected to another page)

Pfcyp51 exclusively determines reduced sensitivity to 14α-demethylase inhibitor fungicides in the banana black Sigatoka pathogen Pseudocercospora fijiensis

Oct 2019 | Chong P, Vichou A, Schouten H J, Meijer H J G, Isaza R E A, Kema G H J,
PLOS ONE. 2019. 14(10) e0223858
DOI: 10.1371/journal.pone.0223858 | Show abstract ...

The haploid fungus Pseudocercospora fijiensis causes black Sigatoka in banana and is chiefly controlled by extensive fungicide applications, threatening occupational health and the environment. The 14α-Demethylase Inhibitors (DMIs) are important disease control fungicides, but they lose sensitivity in a rather gradual fashion, suggesting an underlying polygenic genetic mechanism. In spite of this, evidence found thus far suggests that P. fijiensis cyp51 gene mutations are the main responsible factor for sensitivity loss in the field. To better understand the mechanisms involved in DMI resistance, in this study we constructed a genetic map using DArTseq markers on two F1 populations generated by crossing two different DMI resistant strains with a sensitive strain. Analysis of the inheritance of DMI resistance in the F₁ populations revealed two major and discrete DMI-sensitivity groups. This is an indicative of a single major responsible gene. Using the DMI-sensitivity scorings of both F1 populations and the generation of genetic linkage maps, the sensitivity causal factor was located in a single genetic region. Full agreement was found for genetic markers in either population, underlining the robustness of the approach. The two maps indicated a similar genetic region where the Pfcyp51 gene is found. Sequence analyses of the Pfcyp51 gene of the F₁ populations also revealed a matching bimodal distribution with the DMI resistant. Amino acid substitutions in P. fijiensis CYP51 enzyme of the resistant progeny were previously correlated with the loss of DMI sensitivity. In addition, the resistant progeny inherited a Pfcyp51 gene promoter insertion, composed of a repeat element with a palindromic core, also previously correlated with increased gene expression. This genetic approach confirms that Pfcyp51 is the single explanatory gene for reduced sensitivity to DMI fungicides in the analysed P. fijiensis strains. Our study is the first genetic analysis to map the underlying genetic factors for reduced DMI efficacy.

View paper (you will be redirected to another page)

< 1 2 3 4 5 >»

Scientific Papers and Publications

Mapping Agronomic and Quality Traits in Elite Durum Wheat Lines under Differing Water Regimes

Whole-genome diversity, population structure and linkage disequilibrium analysis of globally diverse wheat genotypes using genotyping-by-sequencing DArTseq platform

Identification of molecular markers for the Pc39 gene conferring resistance to crown rust in oat

Identification of potential candidate genes controlling pea aphid tolerance in a Pisum fulvum high‐density integrated DArTseq SNP‐based genetic map

BACKGROUND

RESULTS

CONCLUSIONS

Springtail phylogeography highlights biosecurity risks of repeated invasions and intraregional transfers among remote islands

Application of DArT seq derived SNP tags for comparative genome analysis in fishes; An alternative pipeline using sequence data from a non-traditional model species, Macquaria ambigua

Morphological and molecular characterization of freshwater prawn of genus Macrobrachium in the coastal area of Cameroon

Unlocking the Genetic Diversity and Population Structure of a Wild Gene Source of Wheat, Aegilops biuncialis Vis., and Its Relationship With the Heading Time

GWAS to Identify Genetic Loci for Resistance to Yellow Rust in Wheat Pre-Breeding Lines Derived From Diverse Exotic Crosses

Pfcyp51 exclusively determines reduced sensitivity to 14α-demethylase inhibitor fungicides in the banana black Sigatoka pathogen Pseudocercospora fijiensis