Home
>
Technology and Resources
>
Scientific Papers and Publications

Scientific Papers and Publications

Jump to:

Morphological and molecular characterization of freshwater prawn of genus Macrobrachium in the coastal area of Cameroon

Dec 2019 | Makombu J G, Stomeo F, Oben P M, Tilly E, Stephen O O, Oben B O, Cheruiyot E K, Tarekegn G M, Zango P, Egbe A E, Ndagyong A, Mialhe E, Ngueguim J R, Mujibi F D N,
Ecol Evol.. 2019. 00 : 1-17.
DOI: 10.1002/ece3.5854 | Show abstract ...

View paper (you will be redirected to another page)

Unlocking the Genetic Diversity and Population Structure of a Wild Gene Source of Wheat, Aegilops biuncialis Vis., and Its Relationship With the Heading Time

Nov 2019 | Ivanizs L, Monostori I, Farkas A, Megyeri M, Mikó P, Türkösi E, Gaál E, Lenykó-Thegze A, Szőke-Pázsi K, Szakács É, Darkó É, Kiss T, Kilian A, Molnár I,
Front. Plant Sci. 2019 November. 10 : 1531.
DOI: 10.3389/fpls.2019.01531 | Show abstract ...

View paper (you will be redirected to another page)

GWAS to Identify Genetic Loci for Resistance to Yellow Rust in Wheat Pre-Breeding Lines Derived From Diverse Exotic Crosses

Oct 2019 | Ledesma-Ramírez L, Solís-Moya E, Iturriaga G, Sehgal D, Reyes-Valdes M H, Montero-Tavera V, Sansaloni C P, Burgueño J, Ortiz C, Aguirre-Mancilla C L, Ramírez-Pimentel J G, Vikram P, ,
Front. Plant Sci. 2019 October. 10 : 1390.
DOI: 10.3389/fpls.2019.01390 | Show abstract ...

Yellow rust (YR) or stripe rust, caused by Puccinia striformis f. sp tritici Eriks (Pst), is a major challenge to resistance breeding in wheat. A genome wide association study (GWAS) was performed using 22,415 single nucleotide polymorphism (SNP) markers and 591 haplotypes to identify genomic regions associated with resistance to YR in a subset panel of 419 pre-breeding lines (PBLs) developed at International Center for Maize and Wheat Improvement (CIMMYT). The 419 PBLs were derived from an initial set of 984 PBLs generated by a three-way crossing scheme (exotic/elite1//elite2) among 25 best elites and 244 exotics (synthetics, landraces) from CIMMYT’s germplasm bank. For the study, 419 PBLs were characterized with 22,415 high-quality DArTseq-SNPs and phenotyped for severity of YR disease at five locations in Mexico. A population structure was evident in the panel with three distinct subpopulations, and a genome-wide linkage disequilibrium (LD) decay of 2.5 cM was obtained. Across all five locations, 14 SNPs and 7 haplotype blocks were significantly (P < 0.001) associated with the disease severity explaining 6.0 to 14.1% and 7.9 to 19.9% of variation, respectively. Based on average LD decay of 2.5 cM, identified 14 SNP–trait associations were delimited to seven quantitative trait loci in total. Seven SNPs were part of the two haplotype blocks on chromosome 2A identified in haplotypes-based GWAS. In silico analysis of the identified SNPs showed hits with interesting candidate genes, which are related to pathogenic process or known to regulate induction of genes related to pathogenesis such as those coding for glunolactone oxidase, quinate O-hydroxycinnamoyl transferase, or two-component histidine kinase. The two-component histidine kinase, for example, acts as a sensor in the perception of phytohormones ethylene and cytokinin. Ethylene plays a very important role in regulation of multiple metabolic processes of plants, including induction of defense mechanisms mediated by jasmonate. The SNPs linked to the promising genes identified in the study can be used for marker-assisted selection.

View paper (you will be redirected to another page)

Pfcyp51 exclusively determines reduced sensitivity to 14α-demethylase inhibitor fungicides in the banana black Sigatoka pathogen Pseudocercospora fijiensis

Oct 2019 | Chong P, Vichou A, Schouten H J, Meijer H J G, Isaza R E A, Kema G H J,
PLOS ONE. 2019. 14(10) e0223858
DOI: 10.1371/journal.pone.0223858 | Show abstract ...

The haploid fungus Pseudocercospora fijiensis causes black Sigatoka in banana and is chiefly controlled by extensive fungicide applications, threatening occupational health and the environment. The 14α-Demethylase Inhibitors (DMIs) are important disease control fungicides, but they lose sensitivity in a rather gradual fashion, suggesting an underlying polygenic genetic mechanism. In spite of this, evidence found thus far suggests that P. fijiensis cyp51 gene mutations are the main responsible factor for sensitivity loss in the field. To better understand the mechanisms involved in DMI resistance, in this study we constructed a genetic map using DArTseq markers on two F1 populations generated by crossing two different DMI resistant strains with a sensitive strain. Analysis of the inheritance of DMI resistance in the F₁ populations revealed two major and discrete DMI-sensitivity groups. This is an indicative of a single major responsible gene. Using the DMI-sensitivity scorings of both F1 populations and the generation of genetic linkage maps, the sensitivity causal factor was located in a single genetic region. Full agreement was found for genetic markers in either population, underlining the robustness of the approach. The two maps indicated a similar genetic region where the Pfcyp51 gene is found. Sequence analyses of the Pfcyp51 gene of the F₁ populations also revealed a matching bimodal distribution with the DMI resistant. Amino acid substitutions in P. fijiensis CYP51 enzyme of the resistant progeny were previously correlated with the loss of DMI sensitivity. In addition, the resistant progeny inherited a Pfcyp51 gene promoter insertion, composed of a repeat element with a palindromic core, also previously correlated with increased gene expression. This genetic approach confirms that Pfcyp51 is the single explanatory gene for reduced sensitivity to DMI fungicides in the analysed P. fijiensis strains. Our study is the first genetic analysis to map the underlying genetic factors for reduced DMI efficacy.

View paper (you will be redirected to another page)

Discovering new alleles for yellow spot resistance in the Vavilov wheat collection

Oct 2019 | Dinglasan E G, Singh D, Shankar M, Afanasenko O, Platz G, Godwin I D, Voss-Fels K P, Hickey L T,
Theor Appl Genet. 2019. 132 : 149-162.
DOI: 10.1007/s00122-018-3204-5 | Show abstract ...

View paper (you will be redirected to another page)

Preliminary Genetic Map of a New Recombinant Inbred Line Population for Narrow-leafed Lupin (Lupinus angustifolius L.)

Oct 2019 | Kozak B, Galek R, Zalewski D, Sawicka-Sienkiewicz E,
Agronomy. 2019. 9(10) : 653.
DOI: 10.3390/agronomy9100653 | Show abstract ...

View paper (you will be redirected to another page)

Molecular identification and chromosomal localization of new powdery mildew resistance gene Pm11 in oat

Sep 2019 | Ociepa T, Okoń S, Nucia A, Leśniowska-Nowak J, Paczos-Grzęda E, Bisaga M,
Theor Appl Genet. 2019.
DOI: 10.1007/s00122-019-03449-3 | Show abstract ...

View paper (you will be redirected to another page)

Selection of Parental Material to Maximize Heterosis Using SNP and SilicoDarT Markers in Maize

Sep 2019 | Tomkowiak A, Bocianowski J, Radzikowska D, Kowalczewski P Ł,
Plants. 2019. 8(9) : 349.
DOI: 10.3390/plants8090349 | Show abstract ...

The chief aim of plant breeding is to improve varieties so as to increase their yield and breeding traits. One of the first stages of breeding is the selection of parental forms from the available gene pool of existing varieties. To date, costly and laborious methods based on multiple crossbreeding and phenotypic selection have been necessary to properly assess genetic resources in terms of productivity, quality parameters, and susceptibility to biotic and abiotic stressors. The often long and complicated breeding cycle can be significantly shortened through selection using DNA markers. To this end, use is made of close couplings between the marker and the locus responsible for the inheritance of the functional trait. The aim of this study was to identify single nucleotide polymorphism (SNP) and SilicoDArT markers associated with yield traits and to predict the heterosis effect for yield traits in maize (Zea mays L.). The plant material used in the research consisted of 19 inbred maize lines derived from different starting materials, and 13 hybrids resulting from crossing them. A two-year field experiment with inbred lines and hybrids was established at two Polish breeding stations on 10 m² plots in a randomized block design with three replicates. The biometric measurements included cob length, cob diameter, core length, core diameter, number of rows of grain, number of grains in a row, mass of grain from the cob, weight of one thousand grains, and yield. The isolated DNA was subjected to DArTseq genotyping. Association mapping was performed in this study using a method based on the mixed linear model with the population structure estimated by eigenanalysis (principal component analysis of all markers) and modeled by random effects. Narew, Popis, Kozak, M Glejt, and Grom were the hybrids used in the study that showed the highest significant heterosis effect in 2013 and 2014. The similarity between parental components determined on the basis of SNP and SilicoDArT marker analysis did not exceed 33%. It was found that the genetic similarity between parental components, determined on the basis of SNP and SilicoDArT markers, reflected their degree of relationship, and correlated significantly with the effect of heterosis. As the results indicate, the parental components for heterosis crosses can be selected based on genetic similarity between parental components evaluated using SNP and SilicoDArT markers, supported with information on the origin of parental forms. Of the markers we analyzed, 76 were selected as being significantly associated with at least six traits observed in 2013 and 2014 at both the Łagiewniki and Smolice stations.

View paper (you will be redirected to another page)

Interspecific Genetic Differences and Historical Demography in South American Arowanas (Osteoglossiformes, Osteoglossidae, Osteoglossum)

Sep 2019 | Souza F H S d, Perez M F, Bertollo L A C, Oliveira E A d, Lavoué S, Gestich C C, Ráb P, Ezaz T, Liehr T, Viana P F, Feldberg E, Cioffi M d B,
Genes. 2019. 10(8) : 693.
DOI: 10.3390/genes10090693 | Show abstract ...

View paper (you will be redirected to another page)

Identification of Markers Associated with Yield Traits and Morphological Features in Maize (Zea mays L.)

Sep 2019 | Tomkowiak A, Bocianowski J, Wolko Ł, Adamczyk J, Mikołajczyk S, Kowalczewski P Ł,
Plants. 2019. 8(9) : 330.
DOI: 10.3390/plants8090330 | Show abstract ...

Association mapping is a powerful approach to detect associations between traits of interest and genetic markers based on linkage disequilibrium in molecular plant breeding. The aim of this study was the identification of single nucleotide polymorphisms (SNPs) and SilicoDArT markers associated with yield traits and morphological features in maize. Plant material constituted inbred lines. The field experiment with inbred lines was established on 10 m² plots in a set of complete random blocks in three replicates. We observed 22 quantitative traits. Association mapping was performed in this study using a method based on the mixed linear model with the population structure estimated by eigenanalysis (principal component analysis applied to all markers) and modeled by random effects. As a result of mapping, 969 markers (346 SNPs and 623 SilocoDArT) were selected from 49,911 identified polymorphic molecular markers, which were significantly associated with the analyzed morphological features and yield structure traits. Markers associated with five or six traits were selected during further analyses, including SilicoDArT 4591115 (anthocyanin coloration of anthers, length of main axis above the highest lateral branch, cob length, number of grains per cob, weight of fresh grains per cob and weight of fresh grains per cob at 15% moisture), SilicoDArT 7059939 (anthocyanin coloration of glumes of cob, time of anthesis—50% of flowering plants, time of silk emergence—50% of flowering plants, anthocyanin coloration of anthers and cob diameter), SilicoDArT 5587991 (anthocyanin coloration of glumes of cob, time of anthesis—50% of flowering plants, anthocyanin coloration of anthers, curvature of lateral branches and number of rows of grain). The two genetic similarity dendrograms between the inbred lines were constructed based on all significant SNPs and SilicoDArT markers. On both dendrograms lines clustered according to the kernel structure (flint, dent) and origin. The selected markers may be useful in predicting hybrid formulas in a heterosis culture. The present study demonstrated that molecular SNP and Silico DArT markers could be used in this species to group lines in terms of origin and lines with incomplete origin data. They can also be useful in maize in predicting the hybrid formula and can find applications in the selection of parental components for heterosis crossings.

View paper (you will be redirected to another page)

< 1 2 3 4 5 >»