Thanks to its dramatic cost advantage over alternative technologies for whole genome profiling, Diversity Arrays Technology (DArT) has experienced over a decade of successful applications in plant genetics and breeding.
At the time of its seminal publication in 2001 (Jaccoud at el) DArT represented “disruptive technology” both in comparison to “low plex” genotyping methods (SSR and AFLP) and the high density, parallel SNP typing platforms. This “disruptive” nature was due to large increase in throughput, independence of DNA sequence information and low cost. The cost advantage compared to “marker-by-marker” methods was dramatic, especially for applications requiring comprehensive genome coverage. The main advantage over SNP panels (e.g. Illumina) stemmed from the very low cost of DArT array development and the low service cost provided with this technology by Diversity Arrays Technology Pty Ltd (DArT PL) and its subsidiaries (Triticarte PL).
A decade later the technology has been established in a broad range of organisms, especially plants, with the most recent count at 127 (http://www.diversityarrays.com).
More recently DArT genome profiling has been modified to take advantage of the dramatic progress in performance of DNA sequencing platforms. This new method, DArTseq, represents one of the genotyping by sequencing technology and builds on DArT PL’s many years of expertise of in applying genome complexity reduction methods to efficiently select low copy fraction of plant genomes.
Sequencing of complexity reduced representations was first proposed a decade ago (e.g. http://www.nature.com/nature/journal/v407/n6803/full/407513a0.html?free), but it was only the most recent sequencers (e.g. Hiseq 2000) that produced sufficient sequencing outputs to deploy GbyS methods effectively for genotyping applications in agriculture.