Sample Submission

We offer a DNA-to-data service: customers provide DNA samples and receive genotypic data.

For DArT assays, 500 - 1000 ng of restriction enzyme grade DNA, resuspended in aqueous solution such as TE at a concentration of 50 - 100 ng/microliter should be submitted.

The preferred format for submission is 94 samples supplied in sealed 96-well microtiter plates, leaving wells G12 and H12 empty. It is critical that you seal the plate very well. We recommend the use of a standard fully skirted, V-bottom well 96-well plate, sealed with strips of 8 caps, applied carefully one by one, wrapped in glad wrap. Alternatively, a 3M adhesive film, when properly applied, taking care of sealing the wells one by one, should work well. Heat-sealable PCR films DO NOT perform unless heat- sealed properly: this is not recommended.

As for all genotyping methods, DNA quality has a major effect on DArT data quality. Therefore, we check the samples on arrival for quantity and quality, on a 0.8% agarose gel.

You can find details about our suggested method of DNA extraction [download PDF 79 Kb]. This method proved to work well for many plant species. There is also alternative method developed by one of our collaborators, which should work equally well [download PDF 85 Kb].

To ensure reliable sample tracking and to protect customer's confidentiality, a sample submission form [download Excel document 65 Kb] should be filled in by the customer for automatic input in our Laboratory Information Management System (LIMS) and emailed to us (samplesDiversityArrays.com).

To avoid leaks and cross contamination of samples during shipment, please follow our recommendations about how to ship DNA samples in [download PDF 72 Kb].

Important for international Customers! Please see the special section on Australian quarantine requirements.

Please, e-mail (samplesDiversityArrays.com) us the name of the courrier company you are using and the tracking number of your shipment.