We recommend using the following protocol prior to sending the samples to us. This will help you receive the best quality data for your samples in less time, preventing you from having to resend those samples of poor quality if any.
DNA quality of the samples is controlled by:
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Incubating 1 ul of DNA in Restriction Enzyme buffer at 37°C for 2 hours.
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Resolve the DNA on a 0.8% agarose gel in 1x TAE buffer.
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Inspect the gel image for your samples. Good quality DNA gives a high molecular weight band on the gel image.
This protocol is a mock digest to detect the presence of buffer-activated (usually Mg dependent) nucleases: no restriction enzyme is used.
Another option for you is to send us the image of the DNA quality control you already have for us to ensure the DNA is of sufficient quality for us to proceed with genotyping.
