DArT markers can be used in Marker Assisted Selection (reference). Using the sequence of the DArT marker of interest, you will need to establish a monoplex assay. This will usually require the sequencing of the genomic region of interest, the identification of an appropriate sequence polymorphism in that region and the design of an assay (nearly always a PCR-based assay) to type this polymorphism.

Before you embark into the significant work required to establish one or a few monoplex assays, you may want to consider carefully your needs in terms of density of information you require. We can provide low-density (100-400 markers) DArT assays customised to your application if the demand for the assays is sufficient. The low-density assay can type a small number of genomic regions of interest together with some framework genotyping useful for quality control.