To perform a DArT assay, we require that you send us about 10-20 µl of an aqueous solution of DNA at 50 to 100 ng per µl, not more. Excessive concentration is detrimental to the assay. If your DNA is concentrated: dilute it.
The assay itself requires less than one microliter but we need a larger volume to reduce problems during shipment and to be able to perform Quality Control.